Can you sterilize liquids contaminated with prions? Can you sterilize media with

Question

Can you sterilize liquids contaminated with prions?
Can you sterilize media with heat sensitive nutrients?
Can you sterilized bedding contaminated with infectious patient fluids?
If so, what method. If not, what treatment is best to reduce pathogens to a safe level? Can you sterilize liquids contaminated with prions?
Can you sterilize media with heat sensitive nutrients?
Can you sterilized bedding contaminated with infectious patient fluids?
If so, what method. If not, what treatment is best to reduce pathogens to a safe level?
Can you sterilize media with heat sensitive nutrients?
Can you sterilized bedding contaminated with infectious patient fluids?
If so, what method. If not, what treatment is best to reduce pathogens to a safe level?

Explanation / Answer

prion are highly resistant to routine sterilization methods. for sterilizing liquid contaminated with prion we can use extended heat sterilization in conjugation of 1M SODIUM HYPOCHLORITE, having 2% available chlorine.

sterilization of heat sensitive nutrients is done on RETORTS, these are steam vessels having temp range of 116-129 degree centigrade. liquid food also sterilized in plate type heat exchanger.

bedding contaminated with infectious fluid of patient should be properly sterilised. dry heat sterilization is the best method. it will give better efficiency if used along with formaldehyde.

the best method of sterilization is using radiation as X ray and gamma rays. as it completely kills the pathogen.

autoclaving at 121 degree C for 15-20 minute at 15psi pressure is sufficient to kill all spore forming bacteria.

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