A phlebotomist collected specimens for this requisition and delivered them to th

Question

A phlebotomist collected specimens for this requisition and delivered them to the specimen processing. One specimen was wrapped in foil: one was in a 37 degree C heat block, and one was a normal draw light-blue-top tube with no special handling that was about two thirds full with a note attached starting that it was a difficult draw. The specimen were correctly labeled. Assuming the specimens were handle properly. Which specimen was wrapped in foil? Why? What process does the foil prevent? ___ Which specimen was in the heat block? Why? ___ Which specimen was in the light blue top? Why? Should the light blue top be accepted for testing? Why or why not?

Explanation / Answer

1) Bilirubin specimen was wrapped in foil. it is wrapped by foil to protect it from the light.  when exposed to light for one hour it will undergo up to a 50% reduction in concentration.

2) Cold agglutinin was in a heat block because this specimen must kept at a body temperature.

3) coagulation specimen (PT ) was in the light blue top tube. The light blue top tube contains sodium citrate which is a anti-coagulant it prevents coagulation by binding calcium. anti-coagulant preserves the labile coagulation factors.

4) No, Complete filling of this tube is essential to obtain accurate results.

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