You are a research assistant in a lab that studies nucleic acids. Your advisor g
ID: 164927 • Letter: Y
Question
You are a research assistant in a lab that studies nucleic acids. Your advisor gave you four tubes for analysis. Each of these tubes differs in its contents by the source of its nucleic acids: mouse cytoplasm (single-stranded RNA), yeast nuclei (double-stranded DNA), rotavirus (double-stranded RNA), and parvovirus (single-stranded DNA). Unfortunately, you forgot which source was in which tube. Using the the approximate nucleotide base composition of each sample given in the table below, determine which samples would be destroyed by a DNase?
Tube 1 32 171 01 32 191 2 34 161 15 o 35 3 30 211 0 26 23 4 33 161 34 0 17Explanation / Answer
Tube 1 contains the DNA of yeast nuclei (double-stranded DNA) as it follows Watson-Crick pairing where the A and T numbers are same and cytosine is almost equal to guanine.
Tube 2 contains mouse cytoplasm (single-stranded RNA) as it contain Uracil and also bases does not follow Watson-Crick pairing
Tube 3 contains parvovirus (single-stranded DNA) as Single-stranded DNA does not follow Watson and crick paring
tune 4 contains rotavirus (double-stranded RNA). Rotavirus genome is made up of double-stranded RNA and to make it stable there should be pairing between Uracil and Adenine and Guanine and cytosine that will ensure that virus has equal almost number of adenine and uracil and guanine and cyotsine.
DNase cleave DNA thus tube containing parvovirus (single-stranded DNA) and yeast nuclei (double-stranded DNA) will degrade upon DNAse treatment
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