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i) Could this RNA be from the beginning of a protein coding region? ii) The midd

ID: 165711 • Letter: I

Question

i)               Could this RNA be from the beginning of a protein coding region?   

                  ii)   The middle?

                  iii)   The end?

The following is a small stretch of nucleotide sequence from a bacteria RNA: 5' GGGCCCUGAUGUAACAUAGAUAGG -3' What is the sequence of the DNA template from which it was transcribed? (indicate polarity). Use the genetic code table in Fig 8.11 of your textbook to determine the amino acid sequences encoded by all three reading frames. Use an asterisk (*) to indicate any stop codons you encounter and continue translating beyond them Nucleotide seq: 5' - GGGCCCUGAUGUA ACAUAGAUAGG3' RF1: RF2: RF3: Would the same information be encoded by the RNA sequence that is complementary to the sequence given above? Considering that the RNA sequence is a fragment of an mRNA, answer the questions below and BRIEFLY (10 words or less) explain your reasoning.

Explanation / Answer

Answer:

mRNA: 5’-GGG CCC UGA UGU AAC AUA GAU AGG-3’

DNA template: 3’-CCC GGG ACT ACA TTG TAT CTA TCC-5’

Peptide:

Reading frame 1: 5’-GGG CCC UGA UGU AAC AUA GAU AGG-3’

Peptide:

Gly-Pro-Stop

Reading frame 2: 5’-GGC CCU GAU GUA ACA UAG AUA GG-3’

Gly-Pro-Asp-Val-Thr-Stop

Reading frame 3: 5’-GCC CUG AUG UAA CAU AGA UAG-3’

Ala-Leu-Met-stop


If the mRNA is complementary to the one given above the amino acid sequence will be different.