Cells need receptors on their surface to recognize and attach to extracellular l

Question

Cells need receptors on their surface to recognize and attach to extracellular ligands, which often can include various glyco-conjugated proteins. An important class of receptors is the selectin family, which bind to sugar-modified proteins (glycoproteins, proteoglycans).  You suspect that one of these selectin proteins called F-selectin is involved in mediating cell adhesion to fibronectin surfaces. You have built two cells lines:

a cell line called MD1 that grows in suspension and that does not express F-selectin and

you have engineered MD1 to stably express F-selectin using retroviral technology - you call these cells MD1FS.

Design an experiment to show that F-selectin can mediate cell adhesion to fibronectin surfaces using these cells. What conditions would you need (controls!) to conclude that F-selectin expression mediates cell adhesion via fibronectin. Make sure to predict the outcome for each of your condition and why.

Explanation / Answer

Thw experiment will use integrin to determine the cell adhesion

1.We will take a solution with MD1 and integrin

2.If it is attached closely then it grows in suspension which does not express f--selectin

3.If it is present on its own position then it exhiits F-selectin

The condition is the attraction and cell adhestion of integrin towards MD1 which may happen or not.

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