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Question

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1- Which of the following is responsible for the change in dynamic instability in mirratimler during mitasis Phosphorylation of a protaln with mlerntubulo srablllz ng acrivhy, which results In decreasad mierotutula stabllry bule des Daphosphorylatlan of a proroln wirh mleroru hule dasrabll Ing Mry. which rosuhs In Inrreased mlrronubula stablley O Dephusprioryleuur uf protein with miurotutule stavilizing auuwi.y, whith results in decreased bule stability. 2 You have a dash of warurala cells culture that are all about to euter autosas (ut is a sym:hrouously replacaturg culture o cells). You add to the dish a drug luat zs a poteut urilubutor of RhoA actuvuty. Two liuurs later you analyze your cells under the epe. Wluch oi tae iollow best describes rug har ymm might obserr All cells appear ra ba In rplophase. All ells appear uu be su netept ese. Around 50% of cells appaar ra ba In anaphasa A Around 909 of th rells have und renne anon rnsle 3 which of the following is an event occmming chzing celhilar ir DNA traEt Raped uptake of cytochrome c by hand densarinn NA e inner wall ot rhe 0 Rupturing of cells. oss of phospholipid tippase activiry. 4 WTuch of the following mutations would be likely to result un forma of S pombe cells that are enlongated and wluch require a long time to couplete mutosus A mutation that abolshes Wec1 activry. ion that in reases activity of C A mutation that ncreases actvrty of CAK. A mutation in CDK that prevents it from being phosphorylated by Vlee hos Cdc25

Explanation / Answer

1. The answer is dephosphorylation of a protein with a microtubule stabilising activity which results in decreased microtubule stability. the intrinsic ability of tubulin molecules to hydrolyze GTP allows each microtubule to regulate its size, rapidly remodelling.

2. When RhoA activity is inhibited, all the cells would be arrested in metaphase.

3. During apoptosis, the DNA is fragmented to produce a DNA ladder.

4. Formation of S. pombe cells that are elongated and require a long time to complete mitosis is due to mutation that abolishes Cdc25 activity.

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