link to image: https://ibb.co/kdmQYv Q7 10. These questions concern analysis of
ID: 167524 • Letter: L
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link to image: https://ibb.co/kdmQYv
Q7 10. These questions concern analysis of the status of the cell cycle in a population of cells in liquid culture. The data were obtained by staining live cells with a cell permeable dye that fluoresceswhen it is bound DNA with analysis of the cells using a FAC5. to Betore attempting the question you should review how this instrument tunctions and the format ct the data it produce Io do so, download the ppt Knowledge Nugget tile entitled "HALS Analysis ot Cel Cycle rom the "Knowledge Nugget tab ot the class website. After reviewing this information, download the pdf file en "FACS QUESTIONLWORKED-EXAMPLE.pdf that Is also locatedIn the "Knowledge Nuggets" tab of the class webslte. Review the worked example that ustrates how to answer these types of estions. After reviewing this Information, attempt the following questions. The same figure of FACS graphs 0.e. 1-#60 shown below s used with each question. qu You are given a nask with liquid medid containing populalion of SporrwEN cells thal have d LEmperalure serisitive mulation ord gene Liet causes complete lass of run lion of 8 ne product Lher strictive temperature. The naskh4dbe nin ubdLingat the permissive temperature before it was given to you, and the cells in the flask were replicating asynchronously with respect to the stage of the cell cycle. The mutated gene in this strain of yeast encodes S-cyclin Which FACS plot (see attached figure) best represents how cells in this flesk would appear following growth for 2 hours at the restrictive temperature INote, assume that FACS plot 1 shows analyss of population of S pombe replicating asynchronously ile each cell vithin a population is at a random stage of the cell cycle) n culture. In the rich media assume the yeast have a cel cycle time of 100 mln, with G1-50 mr 25 #6Explanation / Answer
The option 5th is correct. Sixth figure represents the exact growth of cells under restrictive temperature because there will no or lesser growth will occur. In the question the total time by concluding, all phase times: G1+ S +G2+M = 50+25+15+10= 100 minutes. For 2 hour or 120 minutes period, under restrictive temperature, there the number of cells not increased as the other figures have.
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