Can\'t Figure out #2 and #3. Please help! 2. The table below records the amount

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Can't Figure out #2 and #3. Please help!

2. The table below records the amount of time it takes for fruit flies to become immboile in 95% ethanol. Immobile flies are not removed, to the total number of flies is always 10. Devise a way of calculating mean immbolization time of wild type and mutant flies. Do the calculations, showing your work.

3. You will measure the total protein content of homogenates made from wild type and mutant flies at the end of this lab. If one of the homogenates was not making alcohol dehydrogenase, would you expect that to be detectable in an assay of total protein? Explain your answer.

The table below records the amount of time it takes for fruit flies to become immobile in 95% ethanol. Immobile flies are not removed, so the total number of flies is always 10. Devise a way of calculating mean immobilization time of wild-type and mutant flies. Do the calculations, showing your work. You will measure the total protein content of homogenates made from wild-type and mutant flies at the end of this lab. If one of the homogenates was not making alcohol dehydrogenase, would you expect that to be detectable in an assay of total protein? Explain your answer.

Explanation / Answer

2)

Mean immobilization time for wild-type flies is

(5+6+7+8+9+10+11+12+13)/9 = 9 minutes

Mean immobilization time for mutant flies is

(8+9+10+11+12+13)/6 = 10.5 minutes

As the immobilization of wild-type flies started after 5 minutes, the time taken for all those that were immobilized irrespective of their number are considered for calculating the mean. The same is considered for mutant flies as well from 8th minutes onwards. The immobilization time taken for mutant flies appears to be greater than that of the wild-type flies.

3)

The absence of the alcohol dehydrogenase protein might not be detected qualitatively in the total protein content measurement. But, the quantitative analysis will show the difference in the content of the total protein in the flies that were producing alcohol dehydrogenase from those that do not produce the same. The amount of alcohol dehydrogenase accounts to some extent of the total protein while a qualitative analysis if done in particular for the alcohol dehydrogenase cannot give expected results in its absence. However, if the qualitative analysis is done for the presence of any protein in general can again give positive result irrespective of the presence or the absence of alcohol dehydrogenase as other proteins are available in the homogenate.

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