Can\'t Figure out #2 and #3. Please help! 2. The table below records the amount

Question

Can't Figure out #2 and #3. Please help!

2. The table below records the amount of time it takes for fruit flies to become immboile in 95% ethanol. Immobile flies are not removed, to the total number of flies is always 10. Devise a way of calculating mean immbolization time of wild type and mutant flies. Do the calculations, showing your work.

2) The table below records the amount of time it takes for fruit flies to become immobile in 95% ethanol. Immobile flies are not removed, so the total number of flies is always 10. Devise a way of calculating mean immobilization time of wild-type and mutant flies. Do the calculations, showing your work. Minutes of Total number of immobile Total number of immobile wild-type fruit flies out of 10 purple group mutant fruit flies exposure out of 10 10 10 10 12 10 10 10 13 3) You will measure the total protein content of homogenates made from wild-type and mutant flies at the end of this lab. If one of the homogenates was not making alcohol dehydrogenase, would you expect that to be detectable in an assay of total protein? Explain your answer.

Explanation / Answer

Answer:

Mean immobilization time for wild type and mutant flies can be determined as below:

Multiply the Minute of exposure value with the number of immobilized flies and divide by total number of flies

For wild type: (1*0 + 2*0 + 3*0 + 4*0 + 5*3 + 6*4 + 7*8 + 8*8 + 9*10 + 10*10 + 11*10 + 12*10 + 13*10)/73 = 709/91 = 9.71 minutes

For mutant purple flies: (1*0 + 2*0 + 3*0 + 4*0 + 5*0 + 6*0 + 7*0 + 8*4 + 9*5 + 10*7 + 11*9 + 12*10 + 13*10)/45 = 496/45 = 11.022 minutes

3) If one of the homogenates was not making alchol dehydrogenase then we expect to detect it in an assay of total protein. Because unless the alochol dehydrogenase is not produced, the total protein content of homogenates should remain similar. However if one of the homogenate is not making alcohol dehydrogenase, then the total protein content of that particular homogenate will be low and can be detected in an assay of total protein.

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