Immunoassays are techniques that use immunology to determine the presence of ant
ID: 167672 • Letter: I
Question
Immunoassays are techniques that use immunology to determine the presence of antigens, antibodies, or both. In one type of enzyme-linked immunoassay test, antibodies are attached to a matrix (such as the bottom of a microtiter plate). Next, the sample to be tested (such as patient serum) is added. An antibody-enzyme complex is then added. Finally, the enzyme substrate is added. The enzyme must catalyze a reaction that is visible (such as a color change), meaning that it is possible to see if the antibodies were present by seeing if there is a color change. The development of the colored product is proportional to the concentration of antibody. What would this sort of test be useful for?
A. It would be useful for detecting whether a patient had antibodies for a particular antigen. The second antibody (attached to the enzyme) is not necessary as the enzyme can react directly with the antigen if the antigen is present. B. It would be useful for detecting a virus in a patient's blood, as the antibody on the matrix would bind to the virus. The second antibody (attached to the enzyme) is able to bind because it is specific for the first antibody. C. It would be useful for detecting whether a patient had antibodies for a particular pathogen. The second antibody (attached to the enzyme) is able to bind because it is specific for the antigen. D. It would be useful for detecting a virus in a patient's blood, as the antibody on the matrix would bind to the virus. The second antibody (attached to the enzyme) is able to bind because it is specific for the antigen.Explanation / Answer
The correct answer is :D.It would be useful for detecting a virus in a patient's blood, as the antibody on the matrix would bind to the virus. The second antibody (attached to the enzyme) is able to bind because it is specific for the antigen.
Reason: The antibody on matrix binds to antigen that in turn binds to antibody linked to enzyme and the amount of color is proportional to the antigen antibody complex formed.
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