11. The expression of the BRF1 gene in mice is normally quite low, but mutations
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Question
11. The expression of the BRF1 gene in mice is normally quite low, but mutations in a gene called BRF2 lead to increased expression of BRF1. You have a hunch that nucleosomes are involved in the regulation of BRF1 expression and so you investigate the position of nucleosomes over the TATA box of BRF1 in normal mice and in mice that lack either the BRF2 protein (BRF2-) or part of histone H4 (HHF-) (histone H4 is encoded by the HHF gene). In the table below a normal functional gene is indicated by a plus sign (+).
Which of the following conclusions can be drawn from your data?
a. BRF2 is a repressor of BRF1.
b. BRF2 and HHF are required for the specific pattern of nucleosome positions over the BRF1 upstream region.
c. The specific pattern of nucleosome positioning over the BRF1 upstream region is required for BRF1 repression.
d. BRF2 protein must interact with histone H4.
e. Both a and b
22. Eukaryotic RNA polymerase II alters its conformation after binding to a promoter as it makes the transition from DNA-binding to the transcription of RNA. RNAP II is modified at it’s (C or N)-terminal domain by (methylation or phosphorylation).
a. C, methylation
b. C, phosphorylation
c. N, methylation
d. N, phosphorylation
42. You have an oligonucleotide probe that hybridizes to part of gene A from a eucaryotic cell. Choose whether a cDNA library or a genomic DNA library will be more appropriate to use for the following applications. A) To study the promoter of a gene A. B) To express gene A in bacteria to produce lots of protein A.
a. A; cDNA library, B; cDNA library
b. A; genomic library, B; genomic library
c. A; cDNA library,B; genomic library
d. A; genomic library, B; cDNA library
Explanation / Answer
11.Answer e - BRF2 is a repressor of BRF1 because Mutation of BRF2 lead to over expression of BRF1.BRF2 protein must interact with histone H4 , and this interation lead to supression of BRF1. THE lack of either the BRF2 protein (BRF2-) or part of histone H4 (HHF-) (histone H4 is encoded by the HHF gene) lead to over expression of BRF1.
22 Answer-C
The C terminal Domain (CTD) is Largest subunit of RNA pol II .CTD domain contains the strech of seven amino acids(Try-Ser-Pro-Thr-Ser-Pro-Ser).that is repeated multiple times. And Once they phosphorylated ,the polymerase is able to leave the Pre-initiation complex and begin synthesizing RNA.
42 Answer D
TO study the Promoter you should require non coding sequence which can be only possible in Genomic DNA but not in c DNA because c DNA contains the coding sequece.
To study the gene - require to clone cDNA of gene in vector for over expression because cDNA doesnot contain Intron, its only contain exon.
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