Hallamase is separately digested with V8 protease and cleaved with cyanogen brom

Question

Hallamase is separately digested with V8 protease and cleaved with cyanogen bromide and the following peptides are obtained after each treatment:

V8 protease                                 Cyanogen bromide

ISE                                             TVQRSTKEAF
D                                                VRYCCWDISEDGTPM
TSIVGMVPAE                                      TSIVGM
WAMVRYCCWD                                 VPAEWAM
AF
GTPMTVQRSTKE

Q. Deduce the sequence of hallamase from the above data

Explanation / Answer

TSIVGMVPAEWAMVRYCCWDISEDGTPMTVQRSTKEAF

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