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In qPCR, the relationship between the threshold cycle (CT) and template DNA conc

ID: 173341 • Letter: I

Question

In qPCR, the relationship between the threshold cycle (CT) and template DNA concentration is

the higher the CT value, the higher the concentration of template DNA in your sample

the higher the CT value, the lower the concentration of template DNA in your sample

the lower the CT value, the higher the concentration of template DNA in your sample

the lower the CT value, the lower the concentration of template DNA in your sample

both B and C

both A and D (NOT THIS ONE)

None of the above. There is no relationship between the CT value and template concentration.

Explanation / Answer

The Ct value increases with a decreasing amount of template.
So answer is both B and C
In B and C it they are inversely proportional.

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