Use the following figure to answer the next 3 questions. P3 cat P2 P4 acz Using

Question

Use the following figure to answer the next 3 questions. P3 cat P2 P4 acz Using the figure above when cloning the cat gene into pBLU, you want to identify the orientation in which cat was inserted. Using a PCR action, you use the primer combinations of 1 and 2, 1 and 3, and 1 and 4. You see that there is an expected product size using primers 1 and 3, no product from 1 and 2, and a product from 1 and 4. How did cat insert? n the opposite orientation as lacz Multiple copies were inserted n the same orientation as lacz Nothing was inserted Tries 0/99 Using the figure above, you clone the cat gene into pBLU. To determine the manner in which cat was inserted, you use the primer combinations 1 and 2, l and 3, and 2 and 3. You see a product from every reaction what happened? Multiple copies of cat were inserted Nothing was inserted cat inserted in the same orientation as lacz cat inserted in the opposite orientation as lacz Submit Answer Tries 0/99 Using the figure above when cloning the cat gene into pBLU, you want to identify the orientation in which cat was inserted. Using a PCR action, you use the primer combinations of 1 and 2, 1 and 3, and 1 and 4. You see no product from 1 and 2 and no product from 1 and 3r but you see a smaller product than expected from 1 and 4. How did cat insert? In the same orientation as lacz In the opposite orientation as lacz Nothing was inserted Multiple copies were inserted Submit Answer Tries 0/99

Explanation / Answer

1. If you get a product from primer 1 and 4 and also from 1 and 3, which means that Cat gene was inserted in the same direction as that of LacZ gene.

2. If you see product from all the combinations of the primers, then the Cat gene was inserted in multiple copies.

3. If you see a product smaller than expected when you use a primer combination of 1 and 4, which means that nothing was inserted into the pBLU.

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