Biology Question. Use the following vector map to propose 2 tests to verify that
ID: 174060 • Letter: B
Question
Biology Question.
Use the following vector map to propose 2 tests to verify that the plasmid you have been given is actually pUC-19.
Your friend gives you a pUC-19 empty vector. Use the following vector map to propose 2 tests to verify that the plasmid you have been given is actually pUC-19. DrdI 91 BamBI 51 BstAPI 179 BsmBI 2683 Ndel 183 Eco0109l 2674 Narl fol 235 AatII Tral 2617 Bgli 245 BciVI 2542 Espl 256 Paul 276 Sspl 250 Bmrl 364 2297 387 Apel-EcoRI 396 xmnl 2294 Bogl 2215 Awal Smal TpMI. Minal 412 Scal 2177 Mal 423 Pul 2066 Axel-Hindl Sall 42) wall 2059 PUC19 2,686 bp Pst 435 BorDI 1935 924 lindIII 447 Fspl 1919 Prul 628 Mall 837 NmeA BsalI 659 Bdl 1813 Bprml 1784 Bsp01 Sapl 683 BsrFI 1779 TTil 78 Bsal 1766 AflIII-Pcil 806 1753 Droll 908 Bumrl 1744 Ahdl 1694 BoiVI 1015 Bsell 1110 AlwNI 217 BceAl 1292 PUC19 MICS Sac Sma Xba Sbf Sa Pst Sphl Hind III EcoR Kpn BamH agtgAATTC GAGCTCGG TACCCGGGGATCCTCTA GAGTCGACCTGCAGGIO ATGCAA GCTTGGegtaatcatggtcat 400 410 420 430 440 450 460 S N S S P v R P DE L T S R C A H L S P T I M T M Iacza translational start Sac Smal BssHII Pac Sbf EcoR Kpn Asc BanH agtgAAT Xba Sa Pst Sph Hind 400 410 420 430 440 450 460 470 480 490 S N S S P V R P R A P D K M T M translational startExplanation / Answer
We can isolate puc 19 palsimd contained bacteria by grow them in lactose and ampicillin added medium. Lactose is added because the plasemid has lacZ operon and only this type of bacteria can consume lactose sugar as their energy source. The second is antibiotic ampicillin which is present in plasmid as APR gene. If the bacteria have puc 19, the bacteria will survive in ampicillin condition.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.