NOTE: The answer choices are: A, B, C, D, E for each one. Below is a Restriction
ID: 174176 • Letter: N
Question
NOTE: The answer choices are: A, B, C, D, E for each one.
Below is a Restriction Map of a genomic interval. The location of different restriction enzyme cutting sites are indicated above the line, whereas the distance between adjacent sites is indicated immediately below this in kilobases (kb). Below this are two Southern Blots. Each blot corresponds to the genomic interval cut with the various enzyme combination listed above each lane that was then separated by gel electrophoresis and transferred to nylon membrane. The size marker in kilobases (kb) is given on the left. Each blot shows the hybridization pattern for two different probes from the interval Q4. Based on the fragment sizes on the blots, in which region (A-E) does Probe-1 come from? Q5. Based on the fragment sizes on the blots, in which region (A-E) does Probe-2 come from? Restriction Ma 0.6 1.6 0.4 2.2 1.2Explanation / Answer
Q4. The probe comes from region B (by comparing the bands obtained in southern blots: EcoRI + HindIII and BamHI + HindIII. The 3.2 band of BamHI + HindIII obtained in the blot does not correspond to the actual fragment size in map).
Q5. The probe comes from region D ( if EcoRI + HindIII fragment is taken, which is on the right side of the gene map, it should be ideally giving a band size of 3.4 kb, but the band obtained is 2.6kb)
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