Host restrict on modification system Bue-white selecton for clones Restriction E
ID: 174399 • Letter: H
Question
Host restrict on modification system Bue-white selecton for clones Restriction Endonuclease Multiple cloning Site Antibiotic Resistance cDNA Library Northern Blotting Polymerase Chain Reaction Nested primers: Short Tandem Repeats (STRs) nucleotide nucleotide termination seque 454 lequenc Illumina Sequencing Real Time PCR Consensus sequence Genetic Drift S' Methyl Cap Branch Point RNA editing bE 1) This is a circular, DNA. It is autonomous, and can replicate Ideal vectors to carry DNA. ADE 2) Chemically synthesized smaTaurattide polymers usually shorter than 50 nt and mRNA of a specific gene made by reverse ptase 4) PCR that uses primers are with-in the DNA sequence that has been previously been amplified by another set of primers The primers have a similar name 50 Accumulation of mutations in a gene in the absence of selective pressure occurs when a gene is duplicated and there is no longer functional constraint on the duplicated gene 6) A polynucleotide tail found at the 3' end of all mRNAs or other RNAs that use RNA polymerase ll for transcription 7) A mechanism where gene transcripts can spliced in different ways to produce distinct RNAs that code for tinct 8) Set of paired restriction enzymes a Modification enzymes recognize the same DNA sequence as the restriction enzyme but methylate instead of cutting the DNA groups prevent enzyme from cutting the target sequence. 9) A DNA blotting technique used to show conservation of genes in different species When a probe is hybridized it recognizes (a) band(s) in each lane. 100 A sequence consisting the most common residues to occur at a given site in a set of similar sequences: the sequence represents most common form found bioinformatics alignment 11) A modification mRNA using a guide RNA after transcription but before splcing that alters one (possibly more) codon prior to An enzyme that joins two DN creating a phosphodiester 3 end of one DNA fragment and the 5' ehd another 13) Nucleotide analogs that terminate a polymerizing sequence depriving the growing DNA polymer of a 3' OH group to continue extending of Pley different DNA sequences by performing several PCR amplification reactions all S together in a single tube at the same time. 5 A factor like Sex-lethal that binds in a intron and prevents the splicing of a given 16) A primer for the synthesis of cDNA's using hybridization to a terminal structure found at the 3 end of all mature mRNAs 17 copy of the original DNA region to insert nto a new location. Each time this occurs there is at least a duplication ofnumbers 18) Ribonucleoprotein complexes formed proteins that bind small nudear (snRNA) that are found in the nucleus are the com U1 19) Recom exclusion, inserti nd ultimerization of exons that ca to drive the evolution of new ge represents a single protein d 200 A residue of 7-methylgua the 5 most residue of an RNA throu unique 5,5 triphosphatebond the message from ribaude se digeston and helps initiate traasupodExplanation / Answer
Answer :
Q4. Nested primers
Q5. Gene duplication
Q6. Poly A Tail
Q7. Alternate splicing
Q8. Host restriction modification system
Q11. RNA editing
Q13.Dideoxy nuleotide termination sequenceing or Sanger swquencing method
Q15. Alternative splicing
Q16. cDNA library
Q17. exon shuffling
Q19. Multiple cloning sites
Q20. 5' methyl capping
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