Question XIII. The Western blot (sometimes called the protein immunoblot) is a w

Question

Question XIII. The Western blot (sometimes called the protein immunoblot) is a widely used immunological technique for detecting specific proteins in a sample of tissue homogenate or extract. Using your current knowledge and basic rules of formal and informal logic, please, assess the following statements (170-176) concerning this technique as either factually correct (true) or incorrect (false):

170. Antibodies against the native form are more likely to be used successfully in Western blotting than antibodies against the denatured form of the same antigen.
171. Antibodies against the native form are less likely to be used successfully in Western blotting than antibodies against the denatured form of the same antigen.
172. The fact whether the antibodies were raised against the native or denatured form of an antigen is unlikely to influence their successful usage in Western blotting.
173. Using a polyclonal antibody in Western blotting could be advantageous because it’s likely to react with more epitopes than a monoclonal antibody with the same antigenic specificity.
174. Using a polyclonal antibody in Western blotting could be advantageous because its antigen binding is likely to be stronger than that of a monoclonal antibody with the same antigenic specificity.
175. Using a monoclonal antibody in Western blotting could be advantageous because it’s likely to react with more epitopes than a polyclonal antibody with the same antigenic specificity.
176. Using a monoclonal antibody in Western blotting could be advantageous because its antigen binding is likely to be stronger than that of a polyclonal antibody with the same antigenic specificity.

Explanation / Answer

170. This statement is true. As with antibodies against native form, non denatured form of antigen is preferred.

171. False. As it is the opposite of the above stated statement.

172. This is a false statement. As the antibodies generated against native proteins or antigens are the most effective ones. So it defenitely influence their usage.

173. True. As these antibodies can react to the antigen with more epitopes as compared to the monoclonal antibodies.

174. True. As these antibodies binds to more epitopes and are more tolerant to small changes in the properties, it suggest the strength of the binding to antigens is high.

175. False. For the above stated reasons.

176. False. Reason stated above.

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