When a gene library is to be made for a particular organism, the extracted genom

Question

When a gene library is to be made for a particular organism, the extracted genomic DNA must be cut up into manageable sized pieces with restriction enzymes before being inserted in plasmids (and then forced into bacterial host cells). Why is it necessary that the same restriction enzyme that is used to cut up the genomic DNA also he used to cut open the plasmids that will be used for this work? When a gene library is to be made for a particular organism, the extracted genomic DNA must be cut up into manageable sized pieces with restriction enzymes before being inserted in plasmids (and then forced into bacterial host cells). Why is it necessary that the same restriction enzyme that is used to cut up the genomic DNA also he used to cut open the plasmids that will be used for this work?

Explanation / Answer

Because when a gene and plasmid is cut by a restriction enzyme a staggered cut is being made and sicky ends are produced which can then be ligated if same ends are present. But if same restriction enzyme are not used they result in different sticky ends which upon ligation can not be ligated and thus no recombinants are formed.

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