The following protein gel shows the purification of a protein called FtIspD, whe
ID: 178160 • Letter: T
Question
The following protein gel shows the purification of a protein called FtIspD, where the lane markers in red indicate the following:
Ly = Crude Cellular Lysate
F2 = Fraction 2, Buffer Wash
F3 = Fraction 3, 5 mM Imidazole Wash
F4 = Fraction 4, 10 mM Imidazole Wash
8 = 8 µL of elution product, using 150mM Imidazole
L = Protein Ladder (EZ Rec Ladder from Fisher, shown left in Figure below)
4 – 0.5 = 4-0.5 µL of elution product, using 150mM Imidazole
What is the molecular weight of your protein based on the protein gel (the 10kDa band from the ladder is cut-off)? Size exclusion chromatography of this protein showed a complex weight of roughly 59 kDa. What does that say about the quaternary structure of this protein? Confirm your answers with a literature source, using the full protein name Francisella tularensis cytidylyltransferase as the search terms.
Ly F2 F3 F4 8 L 4 2 1 0.5 1.908 mg/mL 9/2/15 ANH kDa 200 150 L 120 100 85 70 60 50 40 30 25 20 15 10 4-20% SDS-PAGE Coomassie Brilliant Blue R-250 stainedExplanation / Answer
Francisella tularensis 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase: kinetic characterization and phosphoregulation. - in this study it is said the enzyme exists as a dimer.
But the molecular weight mentioned is 29 KD.
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