3. CsrA protein stays in the cell as dimers. Each CsrB RNA binds 22 dimers of Cs

Question

3. CsrA protein stays in the cell as dimers. Each CsrB RNA binds 22 dimers of CsrA and each CsrC RNA binds 9 dimers of CsrA. A cell has 250 dimers of CsrA proteins, ten CsrB RNA, six CsrC RNA and five glgC mRNA. If CsrA have significant higher binding affinity for the binding sites of CsrB RNA and CsrC RNA than the binding sites of glgC mRNA, will CsrA bind any of the glgC mRNAs?
A. Yes
B. No
C. cannot be predicted

6S RNA

4. E. coli cells are rapidly growing in a nutrient rich medium. Under this condition the 6S RNA level will be ____and the sigma _______ will be the major GTF.
A. low/sigma 70
B. high/sigma S
C. low/sigma S
D. high sigma 70

Explanation / Answer

3. B.NO

There will not be any CsrA to bind to glgC mRNA as

10CsrB will require 220 CsrA

6Crs C will require 54CsrA and since there are only 250CsrA, nothing will be left for glgC.

4. D. High sigma70

6S RNA specifically associates with RNA polymerase holoenzyme containing the sigma70 specificity factor,It is the regulator of transcription in E.Coli. Since E.Coli is rapidly growing the transcription rate would also increase thereby increasing the requirement of 6S RNA.

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