You have engineered a gene where you add multiple targeting sequences into the p

Question

You have engineered a gene where you add multiple targeting sequences into the polypeptide sequence. Specifically, you add a ER import targeting sequence at the N-terminus and a nuclear localization sequence (NLS) afterwards. Which statement is correct?

A.The protein will remain in cytoplasm because the multiple sequences will confuse the cell

B.The protein will be directed to the nucleus because the signal peptidase removes the ER sequence

C.The protein will be located in the lumen of the ER because the SRP will direct the polypeptide to the RER for cotranslational import

D.The protein will be localized to the nucleus because the basic amino acids in the NLS is incompatible with transport across the ER membrane

The answer is C. Explain it please!

Explanation / Answer

Often the exposed signal will be identified by the cell proteins first. The ER signal exposed on the N terminal is exposed and thus identified by the SRP. SRP will thus direct it towards ER signal localization downstream pathway. Thus, the answer is C. Only when the ER signal is cleaved, the NLS is exposed and can be recognised.

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