You are a researcher at a small biotech company and your company has just obtain
ID: 179308 • Letter: Y
Question
You are a researcher at a small biotech company and your company has just obtained the license for use of a human GENOMIC DNA fragment putatively encoding a potentially novel protein, which is thought to regulate p53, the known tumor supressor protein. The scientists who originally cloned this GENE fragment HDM5 "claim" that HDM5 shares 90% DNA sequence homology with one of the HDM2 genes (refer to the review Levine & Oren, 2009). They propose that HDM5 may have HDM2-like properties and may be involved in regulating cell proliferation, and thus a good target to potentially develop as a cancer therapy. Your company has asked you to characterize the gene and gene products, as well as to provide an opinion as to its potential human therapeutic uses.
1. You now want to determine the tissue distribution (expression pattern) of your protein HDM5 and compare that to the expression of other HDM2-like proteins.
Explain how you might use one method to determine the relative human tissue expression levels and patterns.
Explanation / Answer
The one method to determine the relative human tissue expression levels and patterns is microarray as discussed in the article by (Kosti,, 2016) “Cross-tissue Analysis of Gene and Protein Expression in Normal and Cancer Tissues” in nature.
This method has lead to the development of a Human Protein Atlas using immunohistochemistry-based tissue microarrays based on 65 major cell types in 45 different normal tissues, (Berglund et al., 2008). The version 6.0 of this publically available database portal contains the protein profile form 8,400 proteins corresponding to approximately 42% of the estimated number of human protein coding genes (Clamp et al., 2007), probably we can find the tissue distribution (expression pattern) of your protein HDM5 and compare that to the expression of other HDM2-like proteins using this atlas
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