5. A hypothetical experiment: A small number of E. coli is transferred into a be

Question

5. A hypothetical experiment: A small number of E. coli is transferred into a beaker containing a solution that includes both glucose (2 mg) and lactose (2 mg) (Figure 1). E. Coli needs energy to reproduce. The estimated number of E. coli in the beaker is measured every few minutes using an optical device, resulting in the following graph (Figure 2).

What would you predict about the levels of glucose and lactose in the solution at each of the numbered points? At which point(s) is the lac operon being expressed (4 points)?

Explanation / Answer

At point 1 in the graph, Glucose in the solution starts getting used.

At point 2, glucose in the media gets depleted completely.

At point 3 galactose enzyme is expressed and starts utilising lactose

At point 4 lactose level are completely depleted.

Lac operon starts is expressed soon after point 2.

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