Genetic experiments have shown that base pairing between U1 snRNA and 5\'-splice

Question

Genetic experiments have shown that base pairing between U1 snRNA and 5'-splice site of mRNA precursor is a. Sufficient for binding b. Necessary but not sufficient for binding c. Unnecessary for binding d. Both A and C Yeast commitment complex has a branch point bridging protein that binds to: a. U1 snRNP protein at the 3'end of the intron b. Mud2p near the 3'end of the intron c. RNA near the 3'end of the intron d. Mud2p near the 5'end of the intron Which of the following produces a female fruit fly? a. An inactive product that allows female-specific splicing of dsx pre-mRNA b. An active product that causes female specific splicing of dsx pre-mRNA c. Female specific splicing of tra transcript d. Both B and C ___commit to splicing at the female specific splice site on the the dsx pre-mRNA. a. Tra b. Tra-2 c. Sr proteins d. All of the above Polyadenylation causes___of pre-mRNA and___of downstream___. a. Cleavage; loss; exons b. Cleavage; loss; introns c. C

Explanation / Answer

34. Answer: A ; Genetic experiments have shown that base pairing between U1 SnRNA and 5' splice site of mRNA preccursor is sufficent for binding.

35. Answer : B ; Yeast commitment complex has a branch point bridging protein that binds to Mud2p near the 3' end of the intron.

36. Answer: D ; An active product that causes female specific spilcing of dsx pre-mRNA and female specific splicing of tra transcript produces female fruit fly.

37. Answer: A ; Tra protein commit to splicing at female specific splice site on the dsx pre-mRNA.

38 Answer : D ; Polyadenylation causes deletion of pre-mRNA and gain of downstream introns

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