Link to figures https://science3492.files.wordpress.com/2016/12/yeast-lab-figure

Question

Link to figures

https://science3492.files.wordpress.com/2016/12/yeast-lab-figures.pdf

Optional substitute for lab report 3.

Answer the following as completely as possible.

We have discussed a and ? strains, mating factor, and its signal transduction pathway.   Sample data of the time spent in each stage of the lifecycle is in the pdf file.

Part A: Explain why in a or ? cultures there are no shmoos or zygotes.

Hypothesize why when mixed the number percentage of haploid nuclei increase.

Part B: Using the above observations, design an experiment to test ONE of the following. Design an experiment means hypothesize, give methods, and predict results if hypothesis is correct).

Put dialysis membrane between cultures.

Filtered supernatant

Placed GTP?S into the cells

Increased and decreased the concentration of one strain

Diluted the media with water.

Explanation / Answer

A. zygotes could be formed when the haploid gametes from two cultures will be mixed. In the above mention experiment, there is no mention of mixing of cultures (a and alpha). So, the zygotes could not be formed.

B. question not clear, design experiment for what?

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