You want to engineer a yeast cell to manufacture and secrete a bacterial protein

Question

You want to engineer a yeast cell to manufacture and secrete a bacterial protein product To do this properly, you need to make certain that the appropriate signal sequence is present. plenty of clathrin-coated pits may be formed. mannose-6-phosphate is added to the protein. the yeast cell is capable of phagocytosis. all of the above A radioactively labeled protein is made by cells and followed through the various organelles in the secretory pathway. After six hours, all of the radioactivity is still primarily in the Golgi apparatus. This suggests that the protein was not translated by the ribosomes. protein does not possess a signal peptide for localization within the lumen of the rough endoplasmic reticulum. protein possesses a signal for localization within the Golgi apparatus. all of the above Uptake of low-density lipoproteins (LDLs) occurs by simple diffusion. bulk-phase endocytosis. facilitated transport. receptor-mediated endocytosis. pinocytosis. A change in a nucleotide sequence that results in addition or deletion of a single nucleotide and largely changes the amino acid sequence of the resulting peptide is known nonsense mutation. silent mutation. missense mutation. frameshift mutation. none of the above The information encoded in DNA is used within a cell in a two-stage process. The two stages of this process are called denaturation and renaturation. base-pairing and denaturation replication and division. transcription and translation. replication and transcription. The base composition in DNA isolated from cow liver cells is 28% adenine; what percent of the bases are cytosine? 14% 22% 28%

Explanation / Answer

54) the answer is (a).. It should have a proper signal sequence of that protein which would help the yeast cell to produce the protein of that sequence.

55) the answer is (d) all of the above

56)*the answer for ldl uptake is receptor - mediated endocytosis..

57) the answer is (c). This type of mutation are called missense mutation.

58) the answer is (d).. The information in DNA is coded in transcription ie RNA formation and translation process ie protein expressions..

59) the answer is (b).. According to the Chargaff's rule ratio of A & G is equal to T& C.. So if A= 28% then G is also 28% total equal to 56%.. Hence the sum of T & C is equal to (100-56) = 44%. Hence Thymine & cytosine is 22% each

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