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An E.coli strain has a mutation in the gene that encodes the Rho protein. Which

ID: 181669 • Letter: A

Question

An E.coli strain has a mutation in the gene that encodes the Rho protein. Which aspect of transcription would you expect to be disrupted in Rho mutants? Initiation Elongation Termination What is the name of the site on the mRNA that gets bound by Rho? T2 site SD site Amino acyl-site Rut site During eukaryotic transcription for RNA polymerase II, the mRNA gets cleaved, leaving part of the mRNA still attached to the DNA/RNA polymerase complex. This RNA is degraded by an endonuclease which eventually allows the RNA polymerase to dissociate from the DNA. What is the name of the endonuclease that performs this function? Rho Helciase Reverse transcriptase Rat 1 What are two general characteristics found in many transposable elements (there are 3 possible answers)? In a single sentence, describe one mechanism by which a transposable element can cause a mutation in a gene. What is the term for transposable elements that are able to move around in the genome on their own? Autonomous transposable elements P-elements SINEs Non-autonomous transposable elements In the datura plant, purple flower color is controlled by a dominate allele P. White flowers are found in plants homozygous for the recessive allele p. Suppose that a purple-flowered datura plant with an unknown genotype is self-fertilized and that its progeny 28 purple flowered plants and 10 white-flowered plants. What is the genotype of the original purple plant? What is the probability that a randomly chosen purple F1 plants is a heterozygote? A gene that controls colorblindness is on the X chromosome. The colorblind allele is recessive to the normal allele. A woman who is a carrier for color has a child with a phenotypically normal man, what is the chance that they will have a non-colorblind girl? A non disjunction event led the couple to have a little boy with Kleinfelter syndrome (XXY) and colorblindness. In which parent did nondisjunction happen? Did nondisjunction happen during meiosis I, meiosis II or is it impossible to tell?

Explanation / Answer

6. Since rho protein is involved in the termination of transcription, so the termination part of the transcription will disrupted in the rho mutant E.coli strain.

7. The name of the site on mRNA that gets bound by rho is RUT site which is called Rho utilisation site.

8. Helicase is the endonuclease that involve in the disruption of DNA-RNA polymerase complex.

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