3) What is the most logical sequence of steps for splicing foreign DNA into a pl

Question

3) What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?

I-Transform bacteria with recombinant DNA molecule.

II-Cut the plasmid DNA using restriction enzymes.

III-Extract plasmid DNA from bacterial cells.

IV-Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.

V-Use ligase to seal plasmid DNA to nonplasmid DNA.

A) I, II, IV, III, V.

B) II, III, V, IV, I.

C) III, II, IV, V, I.

D) III, IV, V, I, II.

E) IV, V, I, II, III.  

Explanation / Answer

Answer:

C) III, II, IV, V, I.

III-Extract plasmid DNA from bacterial cells.--------- II-Cut the plasmid DNA using restriction enzymes------IV-Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments---V-Use ligase to seal plasmid DNA to nonplasmid DNA.------I-Transform bacteria with recombinant DNA molecule.

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