3) What is the most logical sequence of steps for splicing foreign DNA into a pl

ID: 270222 • Letter: 3

Question

3) What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?

I-Transform bacteria with recombinant DNA molecule.

II-Cut the plasmid DNA using restriction enzymes.

III-Extract plasmid DNA from bacterial cells.

IV-Hydrogen-bond the plasmid DNA to foreign DNA fragments.

V-Use ligase to seal plasmid DNA to foreign DNA.

A) I, II, IV, III, V.

B) II, III, V, IV, I.

C) III, II, IV, V, I.

D) III, IV, V, I, II.

E) IV, V, I, II, III.

4) Bacteria containing recombinant plasmids are often identified by which process?

A) examining the cells with an electron microscope.

B) using radioactive tracers to locate the plasmids.

C) exposing the bacteria to an antibiotic that kills cells lacking the resistant plasmid.

D) removing the DNA of all cells in a culture to see which cells have plasmids.

E) producing antibodies specific for each bacterium containing a recombinant plasmid.

3. C III, II, IV, V, I

4. C. Exposing the bacteria to an antibiotic that kills cells lacking the resistant plasmid

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