Can someone help me with these two questions? Integral membrane protein X intera

Question

Can someone help me with these two questions?

Integral membrane protein X interacts directly with the actin cytoskeleton. You transfect cells with a Protein X - GFP fusion or with a mutant Protein-X-GFP fusion that is unable to bind actin. You then perform a FRAP experiment with both sets of transfected cells. Which result would be more likely? A) The Protein X-GFP transfected cells would have recovery of fluorescence, but the Protein X-GFP mutant transfected cells would not. The Protein X-GFP mutant transfected cells would have recovery of fluorescence, but the Protein X-GFP transfected cells would not. B) The Protein X-GFP transfected cells would have recovery of florescence more quickly than the Protein X-GFP mutant transfected cells. C) D) The Protein X-GFP mutant transfected cells would have recovery of florescence more quickly than the Protein X-GFP transfected cells. E)Depending on how strongly actin binding affected Protein X-GFP motility you might predict A or C F) Depending on how strongly actin binding affected Protein X-GFP motility you might predict B or D. Question 9 0/3.5 points Membranes can serve as a scaffold for biochemical activities by: A) allowing some soultes to enter cells, but not others B) spreading enzymatic activity over an entire cellular surface, rather than concentrating it in one area. C) reducing the space in which molecules must move to interact with several membrane proteins 0 D) phospolipids interacting with ATP to actively induce biochemical processes. E) Both B and D are correct.

Explanation / Answer

option A is correct because the normal cells with flourosent protein have colour after FRAP and mutant cells doesnt shows any fluoroscent recovery after photo bleaching.

Option a is correct. The cell membrane is semipermeable hence it allows the transport of specific molecules or ions across the membrane.

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