calculate the final concentration of each of the components in the reaction mixt

Question

calculate the final concentration of each of the components in the reaction mixture described in the folliwing experiment:

The enzyme tyrosinase is found in large concentrations in plant tissues. This enzyme is responsible for the browning of peeled potatoes and bruised fruits. In an assay to determine the activity of the tyrosinase in a potato extract, the following reaction mixture was prepared:

1ml of potato extract, 2ml of 5mM dopa (3,4-dihydroxyphenyalanine), 0.5ml of 20mM citrate buffer, 0.5ml of 1% hydroquinone and 2ml distilled water.
What are the final concentrations of dopa, citrate buffer, and hydroquinone in the reaction mixture?

Explanation / Answer

your total volume of the mixture will be the same for all of them so that's a good place to start: 1ml of potato extract, 2ml of 5mM dopa (3,4-dihydroxyphenyalanine), 0.5ml of 20mM citrate buffer, 0.5ml of 1% hydroquinone 2ml distilled water. you have 6 ml for the mixture use original concentration*original volume/new volume for each: dopa : 5mM*2ml/6ml = 1.67mM citrate buffer: 0.5ml*20mM/6 ml= 1.67mM hydroquinone : 0.5ml*1%/6ml= .083%

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