rou are an undergraduate student that works on earthworms.You have identified a

Question

rou are an undergraduate student that works on earthworms.You have identified a chemical that you call HOOKBACTIN (HBN for short) that causes wild type earthworms to form hooked worms (See Figure below). 1. Wild type Earthworms No HBN Straight) Addition of HBN (Hooked) You are presently taking BIO260 and decide you are going to use mutational analysis to understand how HBN works. To do this you mutagenize wild type earthworms so that you can look for mutants that may identify genes that are responsible for the effects of HBN on earthworm growth

Explanation / Answer

2) mutagenize the eggs of your mutant round worms. If they grow to be a wild type (straight) worm then the mutant is a suppressor of HBN.

3) cross your double mutant worms with single mutant HBN worms. If your second mutation was in some other gene that suppresses the round worm phenotype then it will do the same to the 2nd copy of the HBN and you should get wild type progeny. But if the double mutant is a revertant, then the new HBN copy which it would receive would not be complemented and you would get rounded progenies

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