Homework #2 1. This is a two part problem using this sequence 5\' CGAATGACGACGGG

Question

Homework #2 1. This is a two part problem using this sequence 5' CGAATGACGACGGGCTGAACACAT 3' 3' GCTT ACTGCTGCCCGACT TGTGTA 5' Part I. Somewhere in the above DNA sequence is an open reading frame (ORF...in the steps below, decode this ORF into a polypeptide. i. wrfte-out the mRNA molecule that would be produced if this DNA sequence was transcribed by mRNA polymerase. Clue-the sense strand is the top strand ii. find the series of codons encoded within the ORF in the mRNA sequence and then underline or draw a vertical/line between each codon. Clue:-an ORF begins with a start codon and ends with a stop codon ili. Using the attached codon table, deduce and write-out the linear structure of the polypeptide encoded by the series of codonsbe sure to label the N-terminus and C-terminus of the polypeptide using NH2 and COOH for the N-and C-terminus ends, respectively

Explanation / Answer

Answer:

1. i. DNA: 5' CGAATGACGACGGGCTGAACACAT 3'

mRNA: 5' CGAAUGACGACGGGCUGAACACAU 3'

ii. The codons have been underlined in the mRNA:

5' CGA AUG ACG ACG GGC UGA ACACAU 3'

iii. Polypeptide: NH2 - Met - Thr - Thr - Gly - COOH

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