What is the purpose of using primers targeting PSll sequence for all samples, in

Question

What is the purpose of using primers targeting PSll sequence for all samples, in this experiment? Choose the single, best answer. A Verification that our DNA isolation was successful and gave us enough template DNA for PCR OB. Verification that our DNA isolation was successful but is a fluke because plants do not have DNA OC. Verification that our DNA isolation was successful and indeed comes from a plant DVerification that our DNA isolation was successful and the PSIl gene is present in sufficient copies for PCR QUESTION 3 Why is PCR a good method to detect genetic modifications in organisms? Choose all that apply: OA Even if cells/ proteins are degraded in sample, it can work on extracted DNA B.It is a simple technique and less time consuming C.You can use it on unknown DNA sequence templates/ novel organisms DRequires very little template DNA from test sample

Explanation / Answer

Please find the answers below:

Answer 1: Choice C (While conducting a PCR experiment, a house-keeping gene is always amplified simultaneously to ensure that there is no contamination of the host DNA by external sources. Since PSII genes are found only in plants, this PCR is always performed along with other sets of PCRs in order to ensure that the original DNA sample was pure)

Answer 2: Choices A, C and D (In order to investigate the genetic modifications, the DNA as a sample is required even if proteins and other cells are not available. The PCR uses a very little amount of DNA to give very good results and hence is very reliable. Further, the ability of PCR to compare two or more sequences merely on the basis of binding of probe and strand amplification helps to investigate the differences among original and modified sequences)

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.