Cell & Molec Bio Lab Using the data from the table in the picture, how many diff

Question

Cell & Molec Bio Lab

Using the data from the table in the picture, how many different dyes were resolved on the reversed phase minicolumn? What were their colors?

NAMES: PART 3. (Use this page as part of your lab report) NAME OF "UNKNOWN" DRINK MIX: Read the absorbances of cach of your fractions using the wavelengths indicated in the chart below. You might need to dilute them with some water to get enough volume (2 ml) to read in the spec. Some samples might give very high readings. If so, dilute them and try again. Vortex well to mix in cither case. You will probably have fewer fractions than those listed, maybe just 4 or 5. Don't worry about this. You sce what you see in this, as in everything else!! Please refer to the chart below. 61. Note the color or ea wavelength load flow- through fraction1 fraction 2 fraction 3 fraction 4 fraction 5 (nm): 427 (yellow 5) 10961-0003 | 0.010.174 110100 |-0082 487 (yellow 6) 03 0.006 000l 0-040 0 127 000 504 (Red40) |02all d 003 |0 0011-0001 101411-0002 530 (erythrosine) .250 0.00 602 (Bluc2) .092 0 003 0.002-0.0030.001 0023 0 009 -0 0146 0 006 630 (Blacl)0 203 0 000 0 00 "Load" means a sample that was not put through the minicolumn (the original diluted dye mix). "Flow through" means the liquid that came off the column when you put the diluted dye mix through it. This contains any dyes that "flowed through" the column without sticking to it. "Fraction" is just a way to refer to the tube number, with fraction 1 containing the material that came out ("eluted") first, etc "Elute" means that the molecule comes off the column."Eluate" is the material that cluted (came off).

Explanation / Answer

From the picture it is clear that the reversed phase minicoloumn is the flow through column. Four different colors are evolved in dying. The colors Magneta, Green, Cyan and Blue. Why because when red and green mixed, yellow ellutes.,red and blue ellutes magneta and finally blue-green combine to give Cyan.

Related Questions

Navigate

• Browse (All)
• Browse C
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.