Dilution problems I Please note: for all dilution problems, use only the followi

Question

Dilution problems I Please note: for all dilution problems, use only the following diluent volumes: 0.9 mL 9 mL 9.9 mL Answer the following on separate paper: Problems: 1. You have a broth that contains 5.6 x 10 CFU/mL. How many colonies would you expect to count on a plate if you spread out the volume listed below. Please answer using whole number, not exponent. a. 1.0 mL? b.0.5 mL? 2. Draw two ways of making the following dilutions. Indicate all transfer volumes and diluent volumes. a. 10 dilution b. 10 dilution 3. Using only 9.0 mL and 9.9 mL tubes of diluents, draw two ways of obtaining a 1010 dilution. Indicate all transfer volumes and diluent volumes.

Explanation / Answer

1) a) When 1.0 ml is added from the broth that contains 5.6x103 CFU/ml, to the plate, then,

5.6 x 103 =   5600 colony forming units can be expected to be counted on the plate.

b) For 0.5 ml - 5600/2 = 2800 colony forming units can be expected to be counted on the plate.

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