Dilution practice problems 1. What is the dilution factor when 0.2ml is added to

Question

Dilution practice problems 1. What is the dilution factor when 0.2ml is added to 3.8ml of diluent? 2. So in the above problem all successive tubes would have 3.8 mLs of diluent.nYou would then transfer 0.2 of the initial diluted sample into the next tube, mix transfer 0.2, mix and so on. If you had 4 tubes what would be the final dilution of tube 4? 3. A blood glucose of 800 mg/dL was obtained. According to the Manufacturer the highest glucose result which can be obtained on this particular instrument is 500 mg/dL. The sample must be diluted. Explain how you would do this, give an example, and your final result that you would report.

Explanation / Answer

1) Dilution factor= Final volume/Initial Volume

Final volume=0.2+3.8 = 4 ml

Initial Volume= 0.2 ml

Dilution factor= 4/0.2= 20 (Tube 0)

2) Initial dilution is 1: 20 of the original sample

The next tubes are labeled 1, 2,3 and 4

Dilution of Tube 1= 4/0.2= 20

Multiply the original dilution factor (tube 0) to the dilution factor of Tube 1 to get final dilution.

Tube 1 is 20X 20= 40. Hence, it is 1: 400 dilution of the original sample

Tube 2= 4/0.2=20

Dilution factor of Tube 2= 20 X 20 X 20=8000

Tube 2 is 1:8000 dilution of original sample

Tube 3= 4/0.2=20

Dilution factor of Tube 3= 20 X 20 X 20 X 20 =160000

Tube 2 is 1:160000 dilution of original sample

Tube 4= 4/0.2= 20

Dilution factor of Tube 4= 20 X 20 X 20 X 20 X 20=3200000

Tube 2 is 1:3200000 dilution of original sample

Final dilution factor is 3.2 X 106

Tube 4 is a 1: 3200000 dilution of original sample

3) Initial Glucose= 800 mg/dL

As highest glucose that can be measured is 500 mg/dL, you have to dilute the sample to get a final concentration of not more than 400 mg/dL. A lower concentration should be used, as instruments will show fluctuations in measurement at highest concentration.

A 1: 2 dilution of the sample will give a concentration of 400 mg/dL. For this, 1 ml of sample is added to 1 ml of diluent.

A 1:4 dilution will give 800/4= 200 mg/dL concentration. For this 1 ml of sample is dissolved in 3 ml of diluents.

You can also do a 1: 8 dilution, which will give 100 mg/dL concentration. For this, 0.5 ml of sample can be diluted to 3.5 ml diluent.

Related Questions

Navigate

• Browse (All)
• Browse D
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.