What kind of cells might be isolated in the lymphocyte and blasts population in

Question

What kind of cells might be isolated in the lymphocyte and blasts population in figure B? , Human erythroid, lymphoid, myeloid lineage cells can be isolated from whole blood by differential gradient centrifugation (A). Both peripheral blood mononuclear cells (PBMC/MNC) and larger polymorphonuclear cells can also be analyzed by flow cytometry (B), with simple separation by forward scatter (FSC) and side scatter (SSC). B. t Centrifugation as crude Cell Separation Tool- MNC 1000 preparation 800 Plasma 600 Mononuclear cells (MNC) 50% T cells (CD28 70% CD4, 30% CD8) 5-10% B cells (CD19) 5-10% NK cells (CD16, CD56) 30% monocytes (CD14) Janocyles 400-Graruooylei " Lymphocytes, blast! 200 Debris Polymorphonuclear cells Granulocytes-Neutrophils& eosinophls // 200 400 0 noo 1000 RBC Forward angle light scatter FSC (size)

Explanation / Answer

In figure B light scatter show  

granulocytes (eosinophil and basophil)

monocytes

lymphocytes (T- cell , B-cells-)

blast cells-immature cell

debris show dead and ruptured RBC all other cells' organelles.

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