Chiron corpotarion patented the following DNA sequence because it selectively hy

Question

Chiron corpotarion patented the following DNA sequence because it selectively hybridize with DNA from hepatitis C virus. What is the melting temperature of the complex that would form by hybridization with a much longer strand? 5'-CCTGGTTGCTCTTTCTCTATCT-3'

3.34 Chiron Corporation patented the following DNA sequence because it selectively hybridizes with DNA from hepatitis C virus. What is the melting temperature (Tm) of the complex that would form by hybridization with a much longer strand? 5'-CCTGGTTGCTCTTTCTCTATCT-3

Explanation / Answer

Melting temperature Tm calculated by,

Tm= (a A+b T) X 2 + (c G+d C) X 4

a = number of Adenine in the base pairs / b = number of Thymine in the base pairs

c = number of Guanine in the base pairs / d = number of Cytosine in the base pairs

5'-CCTGGTTGCTCTTTCTCTATCT-3'

a = 1 b = 11 c = 3 d = 7

Tm= (a A+b T) X 2 + (c G+d C) X 4

      = (1 + 11) X 2 + (3 + 7) X 4

       = 24 + 40

       = 64 °C

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