Flow Cytometry BO Flow cytometry, illustrated in Figure P20.71, is a technique u

Question

Flow Cytometry BO Flow cytometry, illustrated in Figure P20.71, is a technique used Nozzle to sort cells by type. The cells are placed in a conducting saline solution which is then forced from a nozzle. Liquid The stream breaks up into small stream droplets, each containing one cell. A metal collar surrounds the stream right at the point where the droplets Cells Laser probe separate from the stream. Charging charging collar the collar polarizes the conducting liquid, causing the droplets to be- come charged as they break off from Deflection the stream. A laser beam probes the plates solution just upstream from the charging collar, looking for the pres- ence of certain types of cells. All Collection droplets containing one particular tubes type of cell are given the same charge by the charging collar. Droplets with other desired types of cells receive a different charge, and droplets with no FIGURE P20.71 desired cell receive no charge. The charged droplets then pass between two parallel charged electrodes where they receive a hori- zontal force that directs them into different collection tubes, depend- ing on their charge.

Explanation / Answer

Ans: Because the droplets are conductors, a droplet positive and negative charges will separate while the droplet is in the region between the deflection plates. When a neutral droplet passes between the plates. The droplet dipole moment will point down the droplet.

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