Which medium would inhibit the growth of rapidly growing gram-negative bacilli i

Question

Which medium would inhibit the growth of rapidly growing gram-negative bacilli in a mixed culture containing staphylococcus (a gram positive oorganism) so that you could obtain a pure culture of staphylococcus? Briefly explain your choice

Explain how mannitol salt functions as both a selective and differential agar.

Actinomycete agar is an enrichment medium. Define enrichment medium and explain how actinomycete agar functions as an enrichment medium.

it is generally accepted that dilution plate counts onto a medium like TSA only reveal about 10% of the bacteria that live in soil. Why might this be?

Explanation / Answer

1). The selective media (allows the growth of a particular bacterial species. For example, the Columbia CNA agar medium allows the growth of only the gram-positive organisms and inhibit the growth of gram-negative organisms. So, it can be used to isolate the Staphylococcus bacteria from the mixed cultured culture containing the gram-negative bacilli. The Columbia CNA agar medium contains colistin and nalidixic acid, which inhibit the growth of gram negative bacteria.

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