5) Using the Fnu4HI CAPS assay, you genotype yourselfor at TAS2R38 and learn tha

Question

5) Using the Fnu4HI CAPS assay, you genotype yourselfor at TAS2R38 and learn that you are homozygous for the AVI/AVI allele. Next, you decide to genotype a sample of DNA extracted from a Neanderthal bone You do not wear gloves while setting up the PCR reaction. You do not perform a negative control reaction. You digest the PCR reaction with Fnu4HI and obtain the result shown below. What can you conclude about the genotype of the Neanderthal (AVI/AVI, AVI/PAV, PAVIPAV)? Explain your answer. 300b 200b 100b 50bp Lane 1, 100-bp ladder. Lane 2, Neanderthal sample.

Explanation / Answer

AVI/AVI

If you are homozygous for the allele then according to RFLP you would show two banda after restriction and if the Neanderthel sample is giving two bands then it must too also be AVI/AVI

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