1. NADH standard samples are prepared using serial (step-wise) dilutions. Calcul

ID: 190820 • Letter: 1

Question

1. NADH standard samples are prepared using serial (step-wise) dilutions. Calculate the concentrations of initial NADH standard stocks (column 2) required to produce the corresponding final concentrations (column 1) if diluting 10 ul of the initial NADH stocks (column 2) into a final assay volume of 1.21 mL. 2. If you were starting with a 50 mm NADH solution, calculate the volumes of NADH (column 3) and H,0 (column 4) needed to produce 100 ul of NADH standard stocks of varying concentration (column 2). Volume of H,0 to add Concentration of initial 1001 Volume of 50 mM NADH ul stock (mm) to add (ul) (ul) Final NADH concentration (uM) 200 150 100 | 24.2 24.2 75 50 25

Explanation / Answer

Final NADH conc (in uM)

Conc of initial 100ul stock (mM)

Vol of 50mM NADH to add (ul)

Vol of water to add (ul)

200

24.2

0.0484

99.9516

150

18.15

0.0363

99.9637

100

12.1

0.0242

99.9758

9.075

0.01815

99.98185

6.05

0.0121

99.9879

3.025

0.00605

99.99395

100