Question
part c please
1. Suppose that someday a Martian bacterium is recovered. After treatment with radiation, some colonies of this bacteriun lose their characteristic green tinge (the mutant colonies are white). When wild type Martian bacterium (green-tinged) are broken open and the resulting cell extract is O used to treat the mutant (white) bacteria, some of the mutant bacteria reacquire the green tingeO of wild type Martian bacteria. To test the nature of Martian genetic material, separate portions of the cell extract are treated with DNase, RNase, proteinase K, and glycosidase, which are enzymes that hydrolyze DNA, RNA, protein, and oligosaccharides, respectively. Cell extract treated with DNase and proteinase K coukd still cause reversion of the white Martian bacteria to green. Cell extract treated with either RNase or glycosidase was unable to cause reversion of white bacteria to green. a. What conclusions can be drawn from these experiments? Analysis of the lot of glycosidase used in the initial experiments revealed the presence of small amounts of proteins other than glycosidase. The treatment with glycosidase was repeated with RNasin included; RNasin is an inhibitor of RNase. b. What was the purpose of this last experiment? c. What would be your conclusion if the inclusion of RNasin in the glycosidase treatment restored the ability of the treated cell extract to cause reversion from white to green? d. What would be your conclusion if the cell extract incubated with glycosidase in the presence of RNasin still was incapable of causing reversion from white to green bacteria? Be precise in your language. For example, DNA and RNA do not revert-bacteria do. I suggest that you make a diagram o the experiment similar in design to the diagrams on p. 50 of the packet You do not need to hand in this diagram; just use it to help your understanding of the experiments
Explanation / Answer
From the given information we can conclude that the bacteria which is giving green tinge is produce either some glycoside or RNA which is helping the white tinge bacteria to produce green tinge.
Glycosidase removes the sugar moiety from the complex sugar.
As RNsain is the inhibitor of RNAase, which prevents the degradation of RNA present in the cell lysate.
When the inclusion of RNasin in the glycosidase treated cell restore the ability to convert white to green, gives us the idea that the material which is responsible for the conversion from white to green tinge is the RNA, not the sugar
