2. Trypsin is a hydrophilic protease and therefore is unable to cross the plasma

Question

2. Trypsin is a hydrophilic protease and therefore is unable to cross the plasma membrane and enter a cell. However, it is capable of digesting the hydrophilic portions of membrane proteins. As a result of these properties, researchers investigating membrane proteins have treated cells with trypsin then separated the proteins by SDS-PAGE to gain insight into the orientation of particular proteins within the membrane. Describe an experiment using this information and the results you would expect with the following types of membrane-associated proteins. (Assume the proteins are single polypeptides.) on the surface on the surface (only) exposed on the surface a. an integral membrane protein with the N-terminus exposed b. an integral membrane protein with the C-terminus exposed c. an integral membrane protein with three hydrophilic loops d. a membrane-associated protein facing the cytoplasm e. an imbedded membrane protein

Explanation / Answer

Answer - A. An intergal membrane protein with the C-terminal exposed on the surface.

Intrgal membrane protein present between the phospho lipid bilayer. In this most of the intergal membrane proteins N-terminal or C-Terminal exposed on the surface of the cell membrane. But trypsin cleaves proteins at the carboxyl side C-terminal side of the amino acids lysine and arginine.

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