1 2) chromatography involves separating proteins by binding them. to ligands. (4
ID: 192327 • Letter: 1
Question
1 2) chromatography involves separating proteins by binding them. to ligands. (4 pts) A) Affinity B) Polyacrylamide C) Size-exclusion D) Ion-exchange E) Electrophoresis 13) pts) involves separating proteins by differences in electrostatic charge. (4 A) Affinity chromatography B) Size-exclusion chromatography C) lon-exchange chromatography D) Gel-filtration chromatography 14) A disadvantage of using bacterial cells to produce eukaryotic proteins is that " (4 pts) A) they are simple to grovw B) their media is inexpensive C) they are most like human cells D) they do not correctly fold all proteins E) they grow quicklyExplanation / Answer
12. The correct option is A (reason : The affinity chromatography technique is widely used for the purification of proteinaceous substances like enzymes, hormones, antibodies and nucleic acids like DNA and RNA. The filling material of the seperating column ligand is done with substances like dextran, polyacrylamide, cellulose etc., which can make a complex with specific protein. During the separation when the protein solution is passed, the protein which is able to form a complex with the ligand is retained by the solid support or matrix in the column while those donot form complex leaves the column first. Then the bound protein is removed from the matrix column by means of passing of a dissolving solution.)
13. The correct option is C. (Reason: The ion- exchange chromatography technique is based on electrostatic interactions between charged protein groups and solid support material or matrix which has an ionic charge opposite to that of the protein to be separated.)
14. The correct answer is D. (Reason: The active eukaryotic protein undergoes different degree of folding and modification like phosphorylation, glycolysation etc events in the ER and Golgi body with the help of various associative enzymes and proteins, which are absent in the prokaryotic setups)
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