1 11 21 31 41 GGCGTATAAA GCGACGACTG TAGACTGATG AGCCTATCCA TGGACGCGCC 51 61 71 81

Question

1                        11                       21                      31 41

GGCGTATAAA GCGACGACTG TAGACTGATG AGCCTATCCA TGGACGCGCC

51                       61                       71                     81                     91

ATGGCCCTGT AAGCGGTGCG ATGCAATAAA ACGCGTATCA GTCATTCAGC

101 111                       121

GTAGTCTGAT GCCAGTCGAC    TGC

a) Write down the corresponding sequences, find them in the sequence above and label them:

TATA box sequence:_________________ (label as TATA)

Polyadenylation signal in corresponding mRNA: ____________(label as Poly-A)

Kozak’ssequence in corresponding mRNA: ________________ (label as Kozak)

Start(initiation) codon in corresponding mRNA: _______ (label as start)

Stop(termination) codon in corresponding mRNA: _______ (label as stop)

b) What region of the mRNA contains the open reading frame that will be translated into protein?

A. 41-101       B. 27-35   C. 51-59 D. 71-77

c) Where is the 3’UTR?

A. 51-101 B. 51-123    C. 63-101 D. 71-81

Please do all, thank you.

Explanation / Answer

GGCGTATAAA GCGACGACTG TAGACTGATG AGCCTATCCA TGGACGC

Tata box starting codon

GCCATGGCCCTGT AAGCGGTGCG ATGCAATAAA ACGCGTATCA GTCATTCAGC

Kozak sequence stop codon Poly A tail

GTAGTCTGAT GCCAGTCGAC    TGC

b. Option A is correct that is 41-101. The translated region starts from starting codon to termination (stop) codon.

c. Option C is correct that is 63-101. 3'UTR (3' untranslated region) begins immediately after stop codon and here stop codon is TAA (UAA in corresponding RNA).

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