4. Sequence of Leucine Enkephalin, a brain opioid peptide. a. Complete hydrolysi

Question

4. Sequence of Leucine Enkephalin, a brain opioid peptide. a. Complete hydrolysis by 6M HCI at 110°C followed by amino acid analysis indicated the presence of G (Gly), L (Leu), F (Phe), and Y (Tyr) in a 2:1:1:1 molar ratio. b. Treatment of the peptide with 1-fluoro-2,4, dinitrobenzene (Sanger's Reagent) followed by complete hydrolysis and chromatography indicated the presence of 2,4- dinitrophenyl derivative of tyrosine. No free tyrosine could be found. Complete digestion of the peptide with chymotrypsin followed by chromatography yielded free tyrosine and leucine with a tripeptide containing Phe and Gly in a 1:2 ratio. c. a. b. C.

Explanation / Answer

we are trying to find the peptide sequence here, and performing different tests to deduce the peptides, so sequences should be like:

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