(2) B-8 What is the advantage of producing cukaryotic recombinant proteins in eu
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Question
(2) B-8 What is the advantage of producing cukaryotic recombinant proteins in eukaryotic cells? a) prokaryotic ribosomes cannot recognize eukaryotic start codons. b) eukaryotic cells are easier and less expensive to maintain than prokaryotic cells. c eukaryotic proteins are more likely to form aggregation complexes in eukaryotic cells d) prokaryotic cells cannot perform post-translational modifications, such as glycosylation. e) prokaryotic cells will mis-translate cukaryotic sequences because they use different codons.Explanation / Answer
Prokaryotes are easy to grow and easy to perform recombinants, but they lack post translational modifications of proteins. This makes the eukaryotic cells more ideal for producing recombinant eukaryotic proteins.
Prokaryotes have a simpler degree of organisation as compared to eukaryotes. They lack the compact machinery involved in post tranlational modification of recombinant proteins.
For example, insulin has two chains bridged by sulpher bonds. Both of the chains are obtained separately from bacteria and bridged artificially because bacteria is unable to link them.
On the other hand, eukaryotic cells are comparatively slow but the end products obtained from them do not require any treatment as they produce the perfect product.
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